25 Cents Flat?

[True]

Recently I've been having an issue where I can have a guitar or bass guitar input, tuned with an electric tuner or with the built-in Reason tuner, playing a perfect E. The pitch editor shows it as a perfect E. But when I play a perfect E on a synth (e.g., a sawtooth on a Subtractor or Thor), the synth sounds flat. Very flat. Like 25-cents flat.

It's hard to imagine that this has been a problem for so long that I haven't noticed, but I'm not sure what I would have changed to cause it. The Master Tune is set to 440. My electric tuner is set to 440. I'm running straight into a Focusrite Scarlett 2i2, which has nothing on it that should affect tuning. I've been using Reason since the Record days and even before, so I'm not a noob to recording. Currently I'm running Reason 11 Suite on Windows 10 with an i7 3.0 GHz processor and 16 GB of RAM, so it shouldn't be a performance issue.

Any ideas?

[nooomy]

Recently I've been having an issue where I can have a guitar or bass guitar input, tuned with an electric tuner or with the built-in Reason tuner, playing a perfect E. The pitch editor shows it as a perfect E. But when I play a perfect E on a synth (e.g., a sawtooth on a Subtractor or Thor), the synth sounds flat. Very flat. Like 25-cents flat.

It's hard to imagine that this has been a problem for so long that I haven't noticed, but I'm not sure what I would have changed to cause it. The Master Tune is set to 440. My electric tuner is set to 440. I'm running straight into a Focusrite Scarlett 2i2, which has nothing on it that should affect tuning. I've been using Reason since the Record days and even before, so I'm not a noob to recording. Currently I'm running Reason 11 Suite on Windows 10 with an i7 3.0 GHz processor and 16 GB of RAM, so it shouldn't be a performance issue.

Any ideas?

Have you checked your midi-keyboard, is the pitch wheel zeroed?

What happens if you take a radical piano standard patch and put a E in the sequencer and play that instead?

[selig]

Is the pitch flat, or does it just sound flat? If you play the synth and guitar at the same pitch at the same time, are they in tune/no beating? What does the tuner show for the synth tuning? Could just be a difference between acoustically vs synthetically generated sounds and how we perceive them...

[True]

Thank you!

Based on the suggestions here, I've concluded that it must be a psychoacoustic issue, possibly combined with the attack of the notes pulling slightly sharp.